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SC Proven Antibody Reagents Product Catalog

SC Proven® antibody reagents

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Use the Product Catalog below to order SC Proven antibody reagent products online. We will confirm your order upon receipt, ship your order as promptly as possible and update you regarding anticipated delivery.

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STEM123

STEM123

Mouse Monoclonal Antibody Specific for Human GFAP

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  • Size 50µg, Volume 100µL, Isotype: lgG1, Form: Unconjugated
  • Cat. No. AB-123-U-050
  • Price:
    £103.00
  • £103.00
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  • Reacts specifically with glial fibrillary acidic protein (GFAP) of human cells. GFAP is an intermediate-filament protein that is highly expressed in astrocytes and other cells of astroglial lineage. Does not cross-react with brain tissue or extracts from mouse or rat. Learn more…

STEM123 Image
  • STEM123 detects human GFAP+ astrocytes differentiated from human neural stem cells in vitro.
  • STEM123 detects human GFAP+ astrocytes differentiated from human neural stem cells transplanted into a mouse brain.

Preparation and Storage

STEM123 is generated from cell culture supernatant in low serum conditions and is purified by protein G chromatography. The antibody is stored in Phosphate Buffered Saline (pH 7.4) containing 0.02% sodium azide. Store at 2-8°C.

Usage/Application

STEM123 has been extensively used to identify astrocytes derived from human neural stem cells transplanted into mice and rats. In vitro and in vivo differentiation of human neural stem cells into astrocytes can be determined by immunohistochemistry (typically using a 1:1000 dilution)1,2 and immunofluorescence (typically using a 1:500 dilution)1,3 using STEM123. It is recommended that the investigator determine optimal conditions for use of STEM123 in their own experiments.

For research use only. Not for use in diagnostic or therapeutic procedures. Not for resale.

  1. Tamaki SJ, et al. Neuroprotection of Host Cells by Human Central Nervous System Stem Cells in a Mouse Model of Infantile Neuronal Ceroid Lipofuscinosis. Cell Stem Cell (2009) 5:310-319
  2. Salazar DL, et al. Human Neural Stem Cells Differentiate and Promote Locomotor Recovery in an Early Chronic Spinal Cord Injury NOD-scid Mouse Model. PLoS ONE (2010) 5:e12272
  3. Cummings BJ, et al. Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice. PNAS (2005) 102: 14069-14074

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