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SC Proven Cell Culture Products Catalog

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NDiff N27-AF

NDiff N27-AF

Animal Component-free Neuronal Supplement

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  • 10mL (100x)
  • Cat. No. SF-NS-02-010-AF
  • Price:
    £206.00

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  • Defined, serum-free and animal component-free cell culture supplement for the derivation, maintenance, expansion and/or differentiation of human and mouse embryonic stem (ES) cells, induced pluripotent stem (iPS) cells and tissue-derived neural stem (NS) cells1-11. Learn more…

    You may also be interested in:

    • NDiff N27 proprietary defined, serum-free cell culture supplement — Cat. No. SF-NS-02-010

Supplement of Choice for Stem Cell Culture

The proprietary defined, serum-free and animal component-free NDiff N27-AF cell culture supplement enables the derivation, maintenance, expansion and/or differentiation of human and mouse embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and tissue-derived neural stem (NS) cells1-11.


    (A) Phase Contrast of Mouse E14 ES cell neural differentiation in
    NDiff N27 supplemented RHB-Basal media.
    (B) Oct4 positive Human ES cells in FGF-2 and NDiff N27-AF supplemented media.
    (C) Human fetal brain-derived NS cells differentiated into Tuj1 (red) and GFAP (green) positive neurons in adherent monoculture with NDiff N27-AF and NDiff N2-AF supplemented
    RHB-Basal media.


Based on the original Brewer B27 formulation,12 applications include*:

  • Derivation, maintenance and expansion of mouse ES and iPS cells
  • Neural differentiation of mouse and human ES cells in monolayer culture
  • Derivation, maintenance and expansion of tissue-derived, adherent mouse and human NS cells
  • Differentiation of mouse and human NS cells into functional neurons
  • Maintenance and expansion of human ES and iPS cells
  • *With addition of further supplements

For optimal performance, use NDiff N27-AF with the SC Proven media product RHB-Basal®. This proprietary basal formulation is also animal component-free and contains no neuronal supplements, thereby enabling you to tailor it to the specific requirements of your cell type through the addition of your choice of supplements.










  1. Ying QL, Smith AG, Defined conditions for neural commitment and differentiation. Methods Enzymol. (2008) 365:327-341.
  2. Ying QL, et al., Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotech. (2003) 21:183-186.
  3. Conti L, et al., Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. (2005) 3(9):e283.
  4. Pollard SM, et al., Adherent neural stem (NS) cells from fetal and adult forebrain. Cereb. Cortex (2006) 16 Suppl 1:112-20.
  5. Conti L, et al., Neural stem cell systems: diversities and properties after transplantation in animal models of diseases. Brain Pathol. (2006) 16(2):143-154.
  6. Nichols J, Ying QL, Derivation and propagation of embryonic stem cells in serum- and feeder-free culture. Methods Mol. Biol. (2006) 329:91-98.
  7. Liu Y, et al., A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells. BBRC (2006) 346:131-139.
  8. Yao S, et al., Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. PNAS (2006)
    103(18):6907–6912.
  9. Hanna J, et al., Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ES cells. PNAS (2010)
    107(20):9222-7.
  10. Yu J, et al., Efficient Feeder-Free Episomal Reprogramming with Small Molecules. PLoS ONE (2011) 6(3):e17557.
  11. Tsutsui H, et al., An optimized small molecule inhibitor cocktail supports long-term maintenance of human embryonic stem cells. Nat. Commun. (2011) 2:167.
  12. Brewer G, et al., Optimized Survival of Hippocampal Neurons in B27-Supplemented Neurobasal™, a New Serum-free Medium Combination. J. Neurosci. Res. (1993) 35:567–57.

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