Supplement of Choice for Stem Cell Culture

The proprietary defined, serum-free and animal component-free NDiff N2-AF cell culture supplement enables the derivation, maintenance, expansion and/or differentiation of human and mouse embryonic stem (ES) cells^1-4 and tissue-derived neural stem (NS) cells^5-7.

• Mouse E14 ES cells differentiated into Tuj 1 positive neurons in adherent monoculture with NDiff N2 supplemented base media.
• Human NS cells differentiated into Tuj 1 positive neurons in adherent monoculture with NDiff N2 supplemented base media.

Based on the original Bottenstein formulation,¹ demonstrated applications include*:

• Derivation, maintenance and expansion of mouse ES cells
• Neural differentiation of mouse ES cells in monolayer culture
• Derivation, maintenance and expansion of tissue-derived, adherent mouse and human NS cells
• Differentiation of mouse and human NS cells into functional neurons
• *With addition of further supplements

For optimal performance, use NDiff N2-AF with the SC Proven media product RHB-Basal^®. This proprietary basal formulation is also animal component-free and contains no neuronal supplements, thereby enabling you to tailor it to the specific requirements of your cell type through the addition of your choice of supplements.