Media of Choice for Adherent & Organotypic Neural Stem Cell Culture

RHB-A is a proprietary, defined, serum-free cell culture medium that has been specifically formulated for the culture of pure populations of adherent human and mouse NS cells. This optimized medium ensures the stable maintenance of NS cell differentiation capacity after prolonged culture in vitro¹.

RHB-A has also been demonstrated to:

• Support the differentiation of human and mouse NS cells into functional neurons, with recorded neuronal differentiation of greater than 40%^1,2,9
• Improve the efficiency of neural differentiation of mouse embryonic stem (ES) cells in monolayer culture with recorded differentiation to greater than 80% neural precursors^4,7,8
• Derive, maintain and expand human glioblastoma stem cell lines⁶
• Culture primary human brain tissue in vitro with radial glia derived neurogenesis⁹

Applications

Neural Cell Culture

For the derivation, maintenance and expansion of adherent human and mouse NS cells^1,3,5, RHB-A should be supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2). Key performance attributes of RHB-A include:

• Maintenance of adherent NS cells at ≥ 99% purity
• Continual propagation of NS cells without loss of neurogenic capacity¹

• Human NS cells
• Mouse NS cells

Neural Differentiation

NS to neurons

Sequential withdrawal of the EGF and the FGF-2 growth factor supplements from RHB-A can enable the efficient differentiation of greater than 40% of adherent human NS and mouse NS cells into neurons^{1, 2}.

Differentiated human NS cells

ES to neurons

The use of RHB-A without growth factor supplements has been shown to enable improved differentiation of mouse ES cells when plated in adherent monolayer conditions^4,7. Efficiencies of greater than 80% differentiation of mouse ES cells into neural precursors have been recorded.

Monolayer differentiation of ES cells to neurons

• 1d
• 2d
• 3d
• 4d
• 7d
• 9d

• ES cells
• ES-derived neurons

Glioblastoma Stem Cell Culture

RHB-A has been demonstrated to support the maintenance and expansion of glioblastoma stem cells when supplemented with EGF and FGF-2⁶.

RHB-Basal

To provide maximum flexibility for your research needs, we also provide a basal formulation of the RHB-A medium called RHB-Basal. This proprietary basal formulation does not contain any neuronal supplements, thereby enabling you to tailor RHB-Basal to suit the specific requirements of your cell type through the addition of your choice of supplements. Learn more…

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