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SC Proven Product Catalog

SC Proven® products

undergo rigorous quality control procedures before release. The SC Proven stamp is your assurance that every product bearing our trademark meets impeccable quality standards.

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STEMmFNS-R

STEMmFNS-R

Purified Mouse Fetal Brain-derived NS Cell RNA

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  • Size 10µg
  • Cat. No. CL-MFN02-010
  • Price:
    £208.00

  • Special Introductory Offer! For a limited time only, save 20% off the standard price!

    £208.00
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  • Comprises RNA affinity purified from the lysate of a single cell culture sample of a mouse ex vivo fetal hind brain-derived neural stem (NS) cell line cultured in proprietary SC Proven RHB-A media supplemented with EGF and bFGF. Under culture conditions in this media, mouse fetal brain-derived NS cells grow as a homogenous (>99%) adherent layer, without loss of their neurogenic capacity1-3.  Learn more…

    STEMmFNS is also available in the following forms:

    • STEMmFNS-D Purified Mouse Fetal brain-derived NS Cell gDNA — Cat. No. CL-MFN01-005
    • STEMmFNS-P Purified Mouse Fetal brain-derived NS Cell Protein — Cat. No. CL-MFN03-020

    We also offer a convenient 'Triplet' kit, containing vials of all three STEMmFNS stem cell lysate fractions (gDNA, RNA and Protein):

Microscope bright field and immuno-cytochemistry images of mouse NS cells propagated in RHB-A plus EGF and bFGF.  

     

     

    Applications

    Purified RNA molecules of greater than 200bp are suitable for applications such as:

    • RT-PCR and qualitative RT-PCR
    • Poly A+ selection and cDNA synthesis
    • Nothern blot analyses
    • Micro-arrays

    Preparation and Storage

    This reagent is derived from the lysate of a homogenous stem cell population by sequential affinity chromatography. The reagent is supplied in the following buffer:

    • RNA in DNase and RNase free H2O with an RNase inhibitor added.
      Store at -20°C.


    Note: Multiple freeze-thawing of all purified lysates should be avoided.

    For research use only. Not for use in diagnostic or therapeutic procedures. Not for resale.

    1. Conti L, et al., Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology (2005) 3(9):e283
    2. Pollard SM, et al., Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex (2006) 16:112-120
    3. Pollard SM, et al., Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience (2008) 38:393:403

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