Applications

The purified gDNA averages between 15-30 kilobases in size, and is suitable for applications such as:

• PCR and qualitative real time RT-PCR
• Southern blot analyses
• Comparative Genome Hybridization (CGH)
• Epigenetic, Genotyping and SNP analyses

Purified RNA molecules of greater than 200bp are suitable for applications such as:

• RT-PCR and qualitative RT-PCR
• Poly A+ selection and cDNA synthesis
• Nothern blot analyses
• Micro-arrays

The purified cellular protein is suitable for:

• 1D and 2D SDS-PAGE and Western blotting under either native or denaturing conditions

Preparation and Storage

These reagents are derived from the lysate of a homogenous stem cell population by sequential affinity chromatography. The reagents are supplied in the following buffers:

• gDNA in 10 mM Tris.Cl, 1mM EDTA (pH 8.5). Store at -20°C.
• RNA in DNase and RNase free H₂O with an RNase inhibitor added.
  Store at -20°C.
• Protein in Laemmli-based buffer, without denaturant and not heated; samples can either be run under native SDS-PAGE, or denaturing SDS-PAGE conditions by adding DTT or β-mercaptoethanol and heating. Store at -20°C.

Note: Multiple freeze-thawing of all purified lysates should be avoided.

For research use only. Not for use in diagnostic or therapeutic procedures. Not for resale.